Figure 8.

Phosphorylation of p38 MAPK/MK2, Akt and NF-kB involved in the anti-inflammation of DHL in vivo. Mice were subjected to an intratracheal injection of LPS (5 mg/kg) and subsequent intraperitoneal injection of solvent or DHL (5 mg/kg), then euthanized and the lung tissues were collected to measure protein expression. (A) The expression levels of specific proteins were evaluated by western blotting. (B–E) The quantitative analysis of the ratios of p -p38/T-p38, p-MK2/MK2, p-Akt/Akt, and p-p65/p65 normalized with β-actin, was performed using ImageJ software. Data represents means ± SEM, n = 6–8, * p < 0.05, ** p < 0.01 and *** p < 0.001.