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. 2019 Apr 20;20(8):1949. doi: 10.3390/ijms20081949

Figure 4.

Figure 4

Blocking STAT3 or STAT6 binding activities by decoy oligodeoxynucleotides (ODNs). THP-1/EV and THP-1/IL-32θ cells were transfected with STAT3 or STAT6 or scrambled decoy ODNs for 24h followed by treatment with PMA (10 nM) for 1 h before being prepared for further analyses. (A) Western blot result of the nuclear protein probed with anti-STAT3 or STAT6 antibodies (n = 3). (B) mRNA expression of IL-13 and IL-13Rα2 conducted by RT-qPCR (n = 3). (C) Relative luciferase activities of IL-13 and IL-13Rα2 promoters (n = 4). Data are shown as mean ± SEM. Statistical significance was analyzed using two-way ANOVA test followed by multiple comparison tests (* p < 0.05). Western blot bands were quantified by Fiji software. Results are representative of single experiments.