(a) Chemical structure of cyclic brain-derived neurotrophic factor (BDNF) mimetic peptide. Representative Cryo-TEM image of nanofibers derived from BDNF peptide amphiphiles (PA) co-assembled at 10 mol.% with E2 PA. β-sheet fibrous materials were obtained via assembly of BDNF PA and E2 PA. (b) Western blot densitometry analysis of phosphorylated tyrosine kinase B (p-TrkB) activation by cells treated with BDNF peptide, E2 PA, E2 + E4, linear BDNF, BDNF PA, and BDNF protein for 6 h in vitro. The BDNF PA-treated group showed comparable activation as cells treated with BDNF protein and significantly higher response relative to all other groups. (c) Representative confocal images of neuronal cells treated with BDNF peptide, E2 PA, BDNF PA, and BDNF protein for 24 h in vitro. Red fluorescence represents the axonal marker, pan-axonal neurofilament protein (SMI312), expressed on the cell surface, while green fluorescence represents the dendritic marker, microtubule associated protein 2 (MAP-2). The nucleus was stained using 4′,6-diamidino-2-phenylindole (DAPI) (blue fluorescence). A notable increase in axon length was observed when cells were treated with BDNF PA or BDNF protein, which suggests maturation of neuronal cells activated by TrkB receptor binding. (d) Raster plots show enhanced electrical activity for cells treated with BDNF PA or BDNF protein over 30 days of in vitro culture. (e) Representative confocal images of neuronal cells cultured on three-dimensional (3D) PA gel scaffolds for one week in vitro. Green fluorescence represents the dendritic marker MAP-2 on the cell surface, while red fluorescence represents the neuronal marker, neuron-specific class III beta-tubulin (Tuj-1). Extended neurites and a homogeneous neuronal network were observed in all gels tested. Furthermore, significantly higher MAP-2 expression was observed for cells cultured on BDNF PA gels or native BDNF + E2 PA gels when compared to other groups. (∗) p < 0.05, (∗∗) p < 0.01, and (∗∗∗) p < 0.001. Adapted with permission from [70]. Copyright 2018, American Chemical Society publishing.