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. 2019 Apr 12;24(8):1450. doi: 10.3390/molecules24081450

Figure 10.

Figure 10

Immobilizing proteins onto peptide nanofibrils via split tag. (a) Graphical representation of non-covalent protein conjugation onto self-assembled peptide nanofibrils using a split protein strategy. Blue: a self-assembling peptide (Ac-(FKFE)2-NH2); red: peptide tag; green: split protein fragments. (b) Fourier-transform infrared (FT-IR) spectra indicating that 10% co-assembled FP1S15/Ac-(FKFE)2-NH2 fibrils and 20% G11P1F/Ac-(FKFE)2-NH2 fibrils (20% G11P1F) adopt a β-sheet secondary structure. (c) Optical density curves of cytidine 2′,3′-cyclic monophosphate hydrolysis catalyzed by self-assembled nanofibrils with different amounts of ribonuclease S tag in the presence of ribonuclease S′. (d) Spectra of green fluorescence produced by self-assembled nanofibrils with different amounts of GFP 11 tag in the presence of GFP 1-10. Control spectra are GFP 1–10 alone (red) and GFP 1–10 + GFP 11 (black). Reproduced from reference [113] with permission from The Royal Society of Chemistry.