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. 2019 Apr 20;20(8):1945. doi: 10.3390/ijms20081945

Figure 2.

Figure 2

Phylogenetic relationships between Type 1 fasciclin domains in plant species. The amino acid sequences of fasciclin domains in FLAs were aligned by Clustal Omega 1.2.2 with the guide of HMM profile of fasciclin domains, and the phylogenetic trees were built by Mega 7 using the Maximum Likelihood (ML) method with 85% partial deletion. Bootstrap analyses with 1,000 replicates were performed for support estimation. The confidence values below 50% were cut off, and the confidence values higher than 70% are shown on nodes. The tree was divided into four major clades: Group B, Group C, Group D1, and Group D2. Plant species from different lineages are shown in different shape. FLAs from A. thaliana are indicated for each clade. The order of fasciclin domains was designated from the N-terminus to the C-teminus (e.g., FLA4.1, FLA4.2, and so on). The conserved motifs (H1, H2, and YH motifs) shown below the tree were found using the MEME web server.