Table 1.
Identification of proteins from B. acuminata petals using MALDI-TOF/TOF-MS.
| Spot No a | Protein Name | Species | Accession No. | MW (kDa)/pI b | Score | Cov c | Fold Changes e | |
|---|---|---|---|---|---|---|---|---|
| Carbohydrate and Energy Metabolism Pathway | ||||||||
| D5 d | malate dehydrogenase, cytoplasmic-like | Solanum lycopersicum | gi|460404529 | 35.361/5.91 | 235 | 25% | −2.33 | −1.55 |
| D10 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase-like | Glycine max | gi|356568270 | 60.799/5.58 | 247 | 11% | −1.92 | −1.32 |
| D19 | 3-isopropylmalate dehydrogenase, chloroplastic-like | Solanum lycopersicum | gi|460386440 | 43.396/6.05 | 232 | 14% | −2.06 | −1.25 |
| U23 d | ATP synthase beta subunit | Eleutherococcus senticosus | gi|343410685 | 39.790/5.77 | 227 | 35% | 1.20 | 1.18 |
| U24 | soluble acid invertase 2 | Orobanche ramosa | gi|294612072 | 61.628/5.24 | 133 | 19% | 2.07 | 1.51 |
| U29 | vacuolar invertase 2 | Gossypium hirsutum | gi|268526570 | 69.303/5.14 | 185 | 8% | 2.16 | 1.98 |
| U32 | ATP synthase subunit beta, mitochondrial-like | Solanum lycopersicum | gi|460382474 | 59.825/5.94 | 869 | 45% | 2.26 | 1.46 |
| U45 | ATP synthase subunit beta, mitochondrial-like | Solanum lycopersicum | gi|460382474 | 59.825/5.94 | 311 | 26% | 3.00 | 2.71 |
| U46 | phosphoenolpyruvate carboxylase kinase 1 | Clusia minor | gi|39842451 | 28.716/6.38 | 88 | 22% | 13.09 | 10.67 |
| U49 | ATP synthase beta subunit | Eleutherococcus senticosus | gi|343410685 | 39.790/5.77 | 227 | 29% | 2.40 | 2.26 |
| U52 | 1,2-beta-fructan 1F-fructosyltransferase | Helianthus tuberosus | gi|3367690 | 69.214/5.02 | 129 | 12% | 2.11 | 2.00 |
| Anthocyanin Metabolic Pathway | ||||||||
| D14 | Anthocyanin-O-methyl transferase | Solanum tuberosum | gi|441433515 | 26.282/5.69 | 90 | 19% | −7.04 | −1.04 |
| D17 | Anthocyanin-5-O-glucosyltransferase | Petunia x hybrida | gi|6683052 | 52.130/5.07 | 98 | 13% | −13.79 | −1.04 |
| D18 | Anthocyanin-5-O-glucosyltransferase | Petunia x hybrida | gi|6683052 | 52.130/5.07 | 100 | 7% | −3.94 | −1.36 |
| Lignin Biosynthesis Pathway | ||||||||
| U41 | caffeate-O-methyltransferase | Liquidambar styraciflua | gi|5732000 | 39.944/5.69 | 80 | 22% | 4.70 | 3.77 |
| U47 | caffeoyl-CoA O-methyltransferase | Broussonetia papyrifera | gi|46394464 | 27.701/5.31 | 412 | 40% | 5.25 | 3.44 |
| Stress Defense and Senescence Proteins | ||||||||
| D1 | polyphenol oxidase E, chloroplastic-like isoform 2 | Solanum lycopersicum | gi|460401035 | 66.181/6.36 | 83 | 18% | −2.66 | −1.02 |
| D2 | polyphenol oxidase | Nicotiana tabacum | gi|92919068 | 57.748/5.92 | 126 | 13% | −2.10 | −1.02 |
| D6 | proteasome subunit alpha type-6-like | Solanum lycopersicum | gi|460412613 | 27.301/6.11 | 102 | 32% | −4.08 | −1.37 |
| U8 | glutathione S-transferase | Solanum commersonii | gi|148616162 | 23.843/5.98 | 82 | 30% | 1.34 | 1.53 |
| D13 | lactoylglutathione lyase-like | Solanum lycopersicum | gi|460373807 | 32.839/5.95 | 283 | 32% | −2.00 | −1.07 |
| U25 | ASR1 protein | Solanum ochranthum | gi|321155417 | 12.547/6.48 | 307 | 34% | 31.33 | 23.55 |
| U28 | CLPC | Theobroma cacao | gi|508775360 | 102.257/6.36 | 373 | 27% | 3.45 | 1.12 |
| U35 | S-adenosyl methionine synthase-like | Solanum tuberosum | gi|78191442 | 43.189/5.52 | 466 | 40% | 2.11 | 2.01 |
| U43 | glutathione S-transferase L3-like | Cicer arietinum | gi|502121795 | 27.092/5.79 | 91 | 15% | 1.59 | 1.51 |
| U44 | putative glutathione S-transferase zeta-class 2 | Brassica napus | gi|330250478 | 25.336/5.53 | 82 | 24% | 2.14 | 1.49 |
| D3 | Adenylosuccinate synthetase, chloroplastic-like | Solanum lycopersicum | gi|460407669 | 55.408/7.55 | 87 | 14% | −2.39 | −1.04 |
| D4 | Adenylosuccinate synthetase1, chloroplastic-like | Solanum lycopersicum | gi|460407669 | 55.408/7.55 | 93 | 18% | −2.11 | −1.38 |
| D15 | Aspartic proteinase | Theobroma cacao | gi|508719874 | 54.428/5.56 | 55 | 3% | −7.85 | −1.08 |
| D16 | endochitinase precursor | Humulus lupulus | gi|4960049 | 33.508/7.42 | 109 | 4% | −3.09 | −1.10 |
| U20 | Small ubiquitin-related modifier 1 | Arabidopsis thaliana | gi|21542462 | 10.969/4.91 | 77 | 48% | 9.19 | 1.68 |
| U26 | annexin p34-like protein-like | Solanum tuberosum | gi|81074127 | 35.909/5.54 | 174 | 24% | 2.01 | 1.59 |
| U27 | Glutamine synthetase 1,4 | Theobroma cacao | gi|508707247 | 39.098/6.02 | 160 | 18% | 5.21 | 2.86 |
| U51 | heat shock 70 protein | Spinacia oleracea | gi|2773050 | 76.094/5.19 | 554 | 25% | 2.02 | 1.07 |
| U53 | Plastid-lipid-associated protein, chloroplast precursor, putative | Ricinus communis | gi|223536371 | 34.979/4.84 | 148 | 24% | 2.06 | 1.78 |
| Floral scent Metabolic Pathway | ||||||||
| U33 | 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase | Ipomoea batatas | gi|325557690 | 51.682/5.90 | 163 | 27% | 2.58 | 2.20 |
| U34 | 1-deoxy-D-xylulose-5-phosphate reductoisomerase | Solanum lycopersicum | gi|350537527 | 51.465/5.94 | 266 | 27% | 2.01 | 1.49 |
| U39 | SAMT | Anthocercis littorea | gi|58201456 | 32.353/4.79 | 151 | 22% | 2.11 | 1.62 |
| U40 | Putative S-adenosyl-L-methionine:Salicylic acid carboxyl methyltransferase | Pisum sativum | gi|37725949 | 40.552/5.17 | 78 | 19% | 15.05 | 7.00 |
| U42 | putative S-adenosyl-L-methionine:Salicylic acid carboxyl methyltransferase | Pisum sativum | gi|37725949 | 40.552/5.17 | 52 | 5% | 2.23 | 1.56 |
| Signaling and Photosynthesis | ||||||||
| D11 | inositol-3-phosphate synthase | Solanum lycopersicum | gi|460388681 | 56.526/5.45 | 189 | 23% | −2.79 | −1.02 |
| D12 | inositol-3-phosphate synthase | Solanum lycopersicum | gi|460388681 | 56.526/5.45 | 318 | 23% | −2.84 | −1.51 |
| U22 | 14-3-3-like protein GF14 Psi | Eutrema salsugineum | gi|309952059 | 28.752/4.78 | 166 | 48% | 2.07 | 1.81 |
| U31 | ruBisCO large subunit-binding protein subunit beta, chloroplastic-like | Cicer arietinum | gi|502125499 | 62.800/5.85 | 348 | 22% | 2.15 | 1.26 |
| Cytoskeleton and Chaperone | ||||||||
| U30 | chaperonin CPN60-2, mitochondrial-like | Solanum lycopersicum | gi|460404682 | 61.521/5.51 | 175 | 18% | 2.37 | 1.99 |
| U36 | beta-actin | Zoysia japonica | gi|284157810 | 41.697/5.23 | 459 | 35% | 2.21 | 2.11 |
| U37 | actin 6 | Populus trichocarpa | gi|222860713 | 40.592/5.05 | 166 | 27% | 1.85 | 1.43 |
| U38 | actin | Gossypium hirsutum | gi|32186904 | 41.878/5.39 | 82 | 27% | 2.5 | 2.25 |
| U50 | 60-kDa chaperonin-60 alpha-polypeptide precursor, partial | Brassica napus | gi|289365 | 57.657/4.84 | 403 | 24% | 2.61 | 1.93 |
| Unclassified Protein | ||||||||
| D7 | predicted protein | Physcomitrella patens subsp. Patens | gi|162667966 | 28.780/5.38 | 79 | 11% | −2.13 | −1.11 |
| D9 | putative transcription factor BTF3-like | Solanum tuberosum | gi|82623431 | 17.472/6.31 | 240 | 34% | −2.06 | −1.12 |
| U48 | cp10-like proteinCP10 | Gossypium hirsutum | gi|21780187 | 26.761/7.77 | 122 | 9% | 1.30 | 1.73 |
a Spot number corresponds to the 2-DE gel in Figure S1; b theoretical molecular mass (MW) and isoelectric point (pI) of the homologous protein calculated with a tool available at NCBInr database.; c sequence coverage; d D down-regulated proteins, U up-regulated proteins.; e ratio of protein levels compared to day 1 (left: Day 3/Day 1, right: Day 5/Day 1).