Figure 1.

B. abortus infect PMNs and promote the exposure of phosphatidylserine. BM cells were incubated with B. abortus-RFP (MOI 50) for 4 h. (A) Cells were mounted using Prolong Gold containing DAPI (blue nuclei). At least 200 PMNs were counted per sample. The percentage of infection and the number of intracellular bacteria (1–5, 6–25, or >25) per cell was determined by fluorescence microscopy. Cell infections were confirmed by flow cytometry. (B) The PMN population analyzed by flow cytometry was gated using anti-Ly6G as PMNs cell marker and analyzed by Annexin V as a cell death marker. These experiments were repeated at least three times.