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. 2019 Apr 12;20(8):1812. doi: 10.3390/ijms20081812

Figure 2.

Figure 2

ELISA screening to identify the specific scFv antibody fragments. Three ELISA’ from each clone were performed. The scFv clones were reacted against B10 and C01 phages, L. infantum SLA and wild-type phage. The scFv-E8 and scFv-C10 clones showed higher reactivity to B10 and C01 phages, respectively. The scFv-E8 and scFv-C10 clones were selected due to the best absorbance ratio between the target and the controls, demonstrating better antibody specificity and a most significant p-value (* p < 0.05; ** p < 0.01, and *** p < 0.005). The x-axis have the clones effectively secreted anti-B10 or C01 and on the y-axis shown the absorbance (492) nm of the samples in ELISA.