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. 2019 Apr 12;20(8):1812. doi: 10.3390/ijms20081812

Figure 4.

Figure 4

Identification of Leishmania β-tubulin protein. The target protein was identified by mass spectrometry (top) and immunoblotting reaction (bottom) against the selected monoclonal antibodies. Immunoblotting experiments using the scFv-E8 and scFV-C10 antibodies were performed for confirmation of the target protein. SDS-PAGE gel followed by Western blot assay confirmed that both monoclonal antibodies recognized the β-tubulin protein, presenting a molecular weight of 49.8 kDa. (1) Protein molecular weight marker (in kDa). (2) The recombinant β-tubulin protein reacting against the anti-Histidine monoclonal antibody. The reaction of the scFv-E8 and scFV-C10 antibodies with the purified protein is shown (in (3) and (4), respectively).