Figure 4.

DH31R regulates the daytime TPR. Comparison of TPR between Dh31r mutant flies (Dh31r^1/Df, red line in (A–C) and heterozygous control flies (Dh31r^1/+, gray line in (A) or Dh31r^Df/+, gray line in (B)) or Dh31r genomic rescue flies (rescue, blue line in (C)). Dh31r mutant flies show an abnormal daytime TPR, in which they prefer a constant temperature of approximately 27°C during the daytime. The control flies exhibit a normal daytime TPR, in which their preferred temperature increases during the daytime (A,B). Genomic rescue of Dh31r flies restored normal daytime TPR (C). Dh31r¹ is a P-element insertion mutant (PBac {WH}Dh31-R^f05546), and Dh31r^Df is a deletion mutant [Df(2R) BSC273]. In the Dh31r genomic rescue fly, the Ch321-57F06 BAC clone, which includes the entire Dh31r gene region, is inserted into the genome. The graphs are modified from Goda et al. 2018, Figure 1 [23]. *: p < 0.05, **: p < 0.01 (comparison to ZT 1–3).