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. 2019 Apr 23;20(8):1991. doi: 10.3390/ijms20081991

Figure 5.

Figure 5

Clobetasol increases proliferation and neurosphere dimension. (a) MTT turnover on NSCs under treatment with glucocorticoids with potential activity on Shh pathway (Clobetasol, Fluticasone and Fluocinonide), and control glucocorticoids treatment (Prednisone, Corticosterone and Dexamethasone, all lacking Smo activity). Data are mean numbers (± SEM) from n ≥ 3 independent experiments. * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001 and **** p-value < 0.0001 vs. control. (b) NSCs rate of cell growth (RCG) after stimulation with glucocorticoids (5.0 μM). Cyclopamine was used to inhibit the effects on the Shh pathway. Data are mean numbers (± SEM) from n ≥ 3 independent experiments. ** p-value < 0.01 vs. untreated control. (c) Violin plot of the NSCs spheres diameter expressed in μm from day 1 to day 4 under Clobetasol treatment in vitro. Scale bar: 50 μm. Data from n ≥ 3 independent experiments. ** p-value < 0.01, **** p-value < 0.0001 vs. day control. Statistical analysis has been performed by using ANOVAs followed by Sidak post-hoc test.