Fig. 2.

GDNF-F-DD is able to rescue the nigrostriatal system. a Immunohistochemical analysis for GDNF, TH, VMAT2, and pRPS6 was performed on brain from animals injected with LV-GDNF-F-DD that were treated with TMP during (Rescue) or after nigrostriatal degeneration (Regeneration). Control animals were injected with LV-GDNF-F-DD and given normal drinking water (OFF). Animals injected with LV-YFP-DD were also treated with TMP during nigrostriatal degeneration (YFP-DD). b Quantification of TH-positive cells in SNpc (n = 7–10 per group). c Quantification of VMAT2-positive cells in SNpc (n = 8–10 per group). d Quantification of pRPS6-positive cells in SNpc (n = 6–9 per group). One-way ANOVA with Tukey multiple comparison tests performed. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.0001. Large scale bar—1200 µm. Medium scale bar—200 µm. Small scale bar—10 µm