Fig. 3.
Transgenic insulin is stored in intracellular granules of AAV2/8-insulin-transduced liver cells. Healthy control (a, left) and AAV2/8-HLP-hINSco-treated (indicated as AAV2/8) (a, right) NOD mouse liver sections stained for insulin. Co-localisation of insulin (red) and C-peptide (green) in liver sections of C57BL/6 mice (b, top panel) and NOD female mice (b, middle panel) injected with 5 × 109 vg AAV2/8-HLP-hINSco after 30 days, and NOD female mice injected with 40 × 109 vg after 218 days (b, bottom panel). Scale bar 50 μm. Magnification of a transduced liver cell in c, where scale bar is 5 μm. Blue shows nuclear staining. Images are representative of at least two experiments