Skip to main content
. 2019 Apr 18;24(8):1534. doi: 10.3390/molecules24081534

Table 4.

Relation between chromatographic behavior of [89Zr]Zr-oxalate preparations and their biodistribution evaluated using ex vivo direct radiometry.

Method [89Zr]Zr-Oxalate
Sample 1 		[89Zr]Zr-Oxalate
Sample 2 		[89Zr]Zr-Oxalate
Sample 3
pH 5.5 pH 7.0 pH 9.0
Chromatography (Radio-TLC)
iTLC-SG/50 mM DTPA (pH 7.0)
Method 1 		graphic file with name molecules-24-01534-i006.jpg graphic file with name molecules-24-01534-i007.jpg graphic file with name molecules-24-01534-i008.jpg
RCP ~ 100% RCP ~ 0% RCP ~ 0%
iTLC-SG/CH3OH-H2O (1:1), 4% TFA (v/v)
Method 2 		graphic file with name molecules-24-01534-i009.jpg graphic file with name molecules-24-01534-i010.jpg graphic file with name molecules-24-01534-i011.jpg
RCP ~ 100% RCP ~ 100% RCP < 30%
Biodistribution (ID/g, %)
Organ/Tissue Time after Intravenous Administration
1 h 24 h 1 h 24 h 1 h 24 h
blood 3.57 ± 0.61 2.19 ± 0.30 3.36 ± 0.60 2.15 ± 0.19 0.99 ± 0.12 0.13 ± 0.02
lungs 1.22 ± 0.29 1.14 ± 0.19 1.54 ± 0.35 1.12 ± 0.09 1.05 ± 0.30 0.17 ± 0.08
heart 1.70 ± 0.16 1.09 ± 0.14 1.42 ± 0.39 1.06 ± 0.11 0.05 ± 0.01 0.11 ± 0.03
stomach 0.47 ± 0.16 0.30 ± 0.05 0.39 ± 0.01 0.25 ± 0.02 0.27 ± 0.07 0.04 ± 0.01
liver 0.68 ± 0.20 0.93 ± 0.05 0.77 ± 0.19 0.88 ± 0.07 5.12 ± 1.81 4.88 ± 1.07
kidney 0.59 ± 0.11 1.52 ± 0.24 0.65 ± 0.16 1.19 ± 0.82 0.27 ± 0.11 0.60 ± 0.29
bladder 0.21 ± 0.01 0.32 ± 0.17 0.34 ± 0.11 0.48 ± 0.22 0.11 ± 0.06 0.06 ± 0.02
s. intestine 0.47 ± 0.02 0.42 ± 0.07 0.52 ± 0.15 0.39 ± 0.06 0.22 ± 0.07 0.06 ± 0.02
l. intestine 0.18 ± 0.01 0.55 ± 0.14 0.22 ± 0.15 0.38 ± 0.11 0.06 ± 0.03 0.10 ± 0.01
muscle 0.11 ± 0.05 0.11 ± 0.01 0.22 ± 0.03 0.14 ± 0.03 0.05 ± 0.01 0.02 ± 0.01
femur 0.26 ± 0.08 0.95 ± 0.25 0.37 ± 0.27 0.97 ± 0.17 0.14 ± 0.02 0.27 ± 0.04