Table 2.

Sample staining and imaging parameters.

Sample Type	Staining	Concentration	Excitation Wavelength	Exposure Time
Fixed cells
	DAPI	2 µg/mL	405 nm	50 ms
	Anti-vimentin	1:100	475 nm	130 ms
	SiR-actin	1 µM	640 nm	50 ms
Live cells
	Nuclear Violet	50 nM	405 nm	50 ms
	SiR-actin	1 µM	640 nm	50 ms