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. 2019 Apr 22;20(8):1972. doi: 10.3390/ijms20081972

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The expression profile in ITB explants and emigrating cells. (A) The main ligament extracellular matrix (ECM) protein collagen type I and the corresponding cell ECM adhesion receptor β1-integrin are shown (A₁: merged, A₂: collagen type I (green), and A₃: β1-integrin (red)). Dashed line: The explant and, on the right side, a thick bundle of collagen fibers are shown. (B) F-actin filament organization and synthesis of vascular endothelial growth factor A (VEGFA) in explants cultured for 5 weeks and emigrating cells (B₁: merged, B₂: F-actin (green), and B₃: VEGFA (red)). (C) The mesenchymal intermediate filament vimentin (red). Scale bars: Figure 6A,C: 100 µm and Figure 6B: 50 µm.