Table 1.
The antibodies used.
| Target | Primary Antibody | Dilution | Secondary Antibody | Dilution |
|---|---|---|---|---|
| Aggrecan | mouse antihuman R&D systems, Minneapolis, USA | 1:30 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| CD44 | mouse-antihuman, Cell signalling Technology, Danvers, USA | 1:50 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| Collagen type I | goat anti human, Abcam, Cambridge, UK | 1:50 | donkey anti goat, Alexa Fluor 488, Invitrogen, Carlsbad, USA | 1:200 |
| Collagen type II | rabbit anti human, Acris Laboratories, Hiddenhausen, Germany | 1:50 | donkey anti rabbit, Alexa Fluor 488, Invitrogen | 1:200 |
| Collagen type III | mouse anti human Acris Laboratories, Hiddenhausen, Germany | 1:30 | donkey-anti-mouse cyanine-3 (cy3), Invitrogen | 1:200 |
| Decorin | rabbit anti human, Acris Laboratories, Hiddenhausen, Germany | 1:30 | donkey anti rabbit, Alexa Fluor 488, Invitrogen | 1:200 |
| Elastin | mouse anti human Acris Laboratories, Hiddenhausen, Germany | 1:30 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| Fibronectin | mouse-antihuman, Dianova, Hamburg, Germany | 1:30 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| β1-integrin | mouse-antihuman, Merck-Millipore | 1:30 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| Lubricin | rabbit-antihuman, Abcam, Cambridge, UK | 1:30 | donkey anti rabbit, Alexa Fluor 488, Invitrogen | 1:200 |
| αSMA | mouse-antihuman, Sigma-Aldrich | 1:50 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| Tenascin C | mouse-antihuman, GeneTex Inc. Biozol, Eching, Germany | 1:30 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| VEGF | mouse-antihuman, R&D Systems | 1:30 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| Vimentin | mouse-antihuman, Dako Cytomation, Hamburg, Germany | 1:50 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |
| Vinculin | mouse-antihuman 1:50, Sigma-Aldrich | 1:50 | donkey-anti-mouse cy-3, Invitrogen | 1:200 |