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. 2019 Apr 12;20(8):1815. doi: 10.3390/ijms20081815

Figure 1.

Figure 1

Western blotting analysis of STAT3 PTMs. The STAT3 PTMs, p-Y705, pS-727 and ac-K685, are detected by immunoblotting of cellular extracts obtained from LNCaP cells both untreated and treated with IL-6 or tBHP. Glut-STAT3 was detected on samples immunoprecipitated with anti-STAT3 antibody. Samples, both untreated and treated with IL-6 or tBHP, were first separated by SDS-PAGE under nonreducing conditions, then subjected to western blot analysis using monoclonal anti-glutathione and anti-STAT3 antibodies.