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. 2019 Apr 15;20(8):1849. doi: 10.3390/ijms20081849

Figure 3.

Figure 3

Promoter assays for PYY, GCG and NTS. The promoter activities of PYY, GCG, and NTS were not up-regulated by IH in Caco-2 cells. Caco-2 cells were transfected with constructs containing the PYY promoter (−2694 to +72), the GCG promoter (−1100 to +125), and the NTS promoter (−2938 to +209) upstream of a firefly luciferase reporter gene in the pGL4.17 vector. After the treatment with normoxia or IH, the luciferase activity was measured. All data are expressed as the mean±SE for each group (n = 6). The statistical analyses were performed using Student’s t-test.