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. 2019 Apr 12;20(8):1810. doi: 10.3390/ijms20081810

Figure 1.

Figure 1

CCNG2 is a target gene of miR-590-3p. (A) miR-590-3p targets CCNG2 3ʹ UTR. Three predicted miR-590-3p binding sites were found in CCNG2 3ʹ UTR. A luciferase reporter construct containing CCNG2 3ʹ UTR was generated using the pMIR-REPORT vector. Transient transfection of miR-590-3p significantly decreased the luciferase activity in ES-2 cells when compared to cells transfected with a non-targeting oligonucleotide (NC). (B) miR-590-3p downregulated CCNG2 mRNA levels. In ES-2 and SKOV3.ip1 cells transiently transfected with miR-590-3p, the CCNG2 mRNA levels were significantly lower than in the control cells transfected with NC. (C) mir-590 inhibited CCNG2 expression. CCNG2 mRNA (left) and protein (right) levels were lower in the ES-2 cells stably overexpressing miR-590-3p precursor, mir-590, than in the control cells expressing the empty vector (EV). Data represent the mean ± SEM (n = 3). Statistical analyses were performed using Student’s t-test. ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. controls.