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. 2019 Apr 12;20(8):1810. doi: 10.3390/ijms20081810

Figure 2.

Figure 2

miR-590-3p targeted FOXO3. (A) A predicted miR-590-3p binding site was found in FOXO3 3ʹ UTR (top). A luciferase reporter assay showed that mir-590-3p significantly decreased the luciferase activity in ES-2 cells (bottom). (B) miR-590-3p decreased the protein level of FOXO3 in SKOV3.ip1 cells. (C) In SKOV3.ip1 and ES-2 cells stably transfected with mir-590, both the mRNA and protein levels of FOXO3 were lower than control cells transfected with an empty vector (EV). (D) ES-2 cells were transfected with siRNA targeting FOXO3. In siFOXO3 transfected cells, FOXO3 mRNA levels were significantly down-regulated. (E) Silencing of FOXO3 reduced CCNG2 mRNA level in ES-2. Data represent the mean ± SEM (n = 3). Statistical analyses were performed using Student’s t-test (A and C), or two-way ANOVA/Student–Newman–Keuls test (D and E). ** p < 0.01, *** p < 0.001, **** p < 0.0001.