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. 2019 Apr 12;20(8):1810. doi: 10.3390/ijms20081810

Figure 3.

Figure 3

Silencing of CCNG2 or FOXO3 mimicked the effects of miR-590-3p. (A) ES-2 and SKOV3.ip1 cells were transiently transfected with a siRNA-targeting CCNG2 and a non-targeting oligonucleotide (NC). Real-time PCR confirmed the down-regulation of CCNG2 mRNA after siCCNG2 transfection. (B) Transient transfection of siCCNG2 and siFOXO3 into ES-2 cells reduced the protein levels of CCNG2 and FOXO3, respectively. (C) ES-2 cells were transiently transfected with siCCNG2, siFOXO3, miR-590-3p, or NC, and cell numbers were determined at 48 h after transfection. (D) SKOV3.ip1 cells were transiently transfected with siCCNG2, siFOXO3, miR-590-3p, or NC, and cell migration was determined. siCCNG2, siFOXO3, and miR-590-3p all significantly increased the number of migrated cells when compared to NC. (E) ES-2 cells were transiently transfected with siCCNG2, siFOXO3, miR-590-3p, or NC. Silencing of CCNG2 or FOXO3, or the overexpression of miR-590-3p induced cell invasion. Red scale bar (D and E) = 25µm. Data represent the mean ± SEM (n = 3). Statistical analyses were performed using Student’s t-test (A), or one-way ANOVA/Student–Newman–Keuls test (C, D, and E). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.