mAb	Monoclonal antibody
CDR	Complementarity determining regions
SHM	Somatic hypermutation
scFv	Single chain variable fragment
Fab	Antigen binding fragment
Fv	Variable fragment
GlySer	Glycine-serine linker
ssDNA	Single-stranded deoxyribonucleic acid
HC	Heavy chain
LC	Light chain
PCR	Polymerase chain reaction
RCA	Rolling circle amplification
dNTPs	Deoxyribonucleoside triphosphates
SM	Saturation mutagenesis
TAG	Amber stop codon
dsDNA	Double-stranded deoxyribonucleic acid
OE-PCR	Overlap extension polymerase chain reaction
sdAbs	Single domain antibodies
ISM	Iterative saturation mutagenesis
B-FIT	B-factor iterative test
TNF	Tumour necrosis factor
UV	Ultraviolet
HPLC	High performance liquid chromatography
PAGE	Polyacrylamide gel electrophoresis
NGS	Next generation sequencing