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. 2019 Apr 30;2019:1897820. doi: 10.1155/2019/1897820

Figure 5.

Figure 5

CA decreased LPS-induced proinflammatory cytokine release in bMEC. The effect of CA on the release of TNF-α, IL-1β, IL-6, and IL-8 in LPS-stimulated bMEC. Cells were challenged with LPS (50 μg/mL) for 12 h after incubation with indicated concentrations (10, 25, and 50 μg/mL) of CA or serum-free media for 3 h; subsequently, the cell culture supernate was prepared. The protein levels of TNF-α, IL-1β, IL-6, and IL-8 were quantified using ELISA kits; the data are the means ± Sem (n = 4). ##P < 0.01 vs. the control group, ∗P < 0.05 vs. the group treated with LPS only, and ∗∗P < 0.01 vs. the group treated with LPS only.