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. 2019 Apr 22;20(8):1960. doi: 10.3390/ijms20081960

Figure 5.

Figure 5

Luciferase assays of promoter activities of RETN, TNFα, and CCL2 in SW872 cells. Reporter plasmids prepared by inserting the promoter fragments of RETN (−979~+20), TNFα (−966~+19), and CCL2 (−3455~+25), upstream of a firefly luciferase reporter gene in pGL4.17 vector were transfected into SW872 cells. After cells were exposed either to IH or normoxia for 24 h, the cells were lysed and the promoter activities of RETN, TNFα, and CCL2 were measured. All data are represented as the mean ± SE of the samples (n = 5–6). The statistical analyses were performed using Student’s t-test.