Figure 5.

EGFR Controls Hair Lineage Transcription Factor Expression

(A) IHC staining of GATA-3 from P10 control and Egfr^Δep mice.

(B) Immunofluorescence staining for P-Cadherin (green), LEF1 (red), and nuclei (blue) from P10 control and Egfr^Δep mice. Arrowheads point to hair shaft cells.

(C) Immunofluorescence staining for ITGB4 (green), IRS and medulla marker AE15 (red), and nuclei (blue) from control and Egfr^Δep mice at P10. Arrowheads point on medulla cells.

(D) Model summarizing TP53-dependent mechanisms of cell death in IFE, ORS, and medulla during the growth phase of HF morphogenesis in Egfr^Δep mice.

(E) Model summarizing TP53-independent effects of EGFR deficiency in epithelial hair lineages.

Scale bars, 20 μm unless otherwise stated.