Figure 6.

Loss of EGFR in Adult HFSCs Results in Growth Delay

(A) Photographs of shaved back skin from control and Egfr^ΔLGR5 mice before and during anagen of second hair cycle.

(B) Quantification of percentage of anagen HFs in mice of indicated genotypes at P88. Bars show analysis of 10 hair follicles per mouse from n = 7–8 mice per genotype. Data are represented as mean ± SD. p values less than 0.05 were considered significant, with ***p < 0.001 as determined by Student's t test.

(C) Quantification of CD45⁺ cells in P88 control and Egfr^ΔLGR5 mice. Bars show analysis of n = 3–5 mice per genotype. Data are represented as mean ± SD. p values less than 0.05 were considered significant. The data sets do not show staticstical significance as determined by Student's t test.

(D) Heatmap of significantly deregulated genes between P88 control and Egfr^ΔLGR5 mice. Each column represents one mouse.

(E) Graphical representation of qRT-PCR validation of randomly selected target genes from LGR5⁺ cells of P88 control and Egfr^ΔLGR5 mice. Bars show analysis of n = 4 mice per genotype. Data are represented as mean ± SD. p values less than 0.05 were considered significant, with *p < 0.05, **p < 0.01 as determined by Student's t test.

(F) Bar diagram of GO terms of significantly deregulated genes.

(G) Heatmap of deregulated genes belonging to GO Term “growth.” Each column represents one mouse.

(H) Model depicting the effects of EGFR in adult HFSCs.

See also Figure S5 and Tables S2A–S2F.