Figure 2.

Aquaporins (AQPs) expression. (A) Expression of AQPs genes in HaCaT cells treated with propolis. The mRNA quantity of several AQPs was determined by qRT-PCR and is expressed as mean relative expression ± SD (n=3). The statistical difference was determined by a two-way ANOVA followed by a Bonferroni post-test (*** p < 0.001). (B) Aquaporin-3 (AQP3) protein expression in HaCaT cells after propolis exposure. Blots representative of two were shown. Lanes were loaded with 30 μg of proteins, probed with anti-AQP3 rabbit polyclonal antibodies and processed as described in the Materials and Methods section. The same blots were stripped and re-probed with anti-beta-2-microglobulin (B2M) polyclonal antibody, as housekeeping. A major band of about 32 kDa was observed (* p < 0.001, t-test).