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Agrobacterium rhizogenes-mediated hairy root cultures in Ligularia fischeri. (a) Hairy roots induction, (b) Hairy root cultures in hormone-free liquid MS medium, c. PCR analysis of the rolC gene in the transgenic root lines. DNA ladder marker lane M, pRiKCTC2703 DNA C(+), transgenic root lines (HRs) induced by A. rhizogenes L1–L6, roots from a non-transgenic plant (NTRs) C(−).
