Table 2.
Kinetic parameters of the wild-type and mutant AHAIRs for NADH or NADPH a.
| AHAIR | NADH | NADPH | ||||
|---|---|---|---|---|---|---|
| Km (μmol L−1) | kcat (s−1) | kcat/Km (s−1/mmol L−1) | Km (μmol L−1) | kcat (s−1) | kcat/Km (s−1/mmol L−1) | |
| Wild-type | 256 ± 13 | 0.93 ± 0.05 | 3.63 | 6.16 ± 1.34 | 0.6 ± 0.03 | 97.40 |
| Mutant | 276 ± 16 | 0.74 ± 0.03 | 2.68 | 67 ± 7 | 0.03 ± 0.0012 | 0.45 |
a The α-acetolactate concentrations were 10 mmol L−1 and variable amounts of NAD(P)H were present. All data represent values of three determinations of triplicate independent experiments with ±SD.