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. 2019 Apr 23;24(8):1595. doi: 10.3390/molecules24081595

Figure 5.

Figure 5

G75-mediated CTGF production through the GR pathway in fibroblasts. (A) G75 competitively bound to GR. DEX was used as positive control. Fluorescent polarization is expressed in millipolarization (mP). Data represent the mean from three independent experiments. (B) G75 induced GR translocation into the nucleus. Fibroblasts were incubated with G75 (5 µM) or DEX (1 µM) for 24 h and immunostained with GR antibody (red) and nucleus was stained with DAPI (blue). Original magnification was 400×. Relative GR translocation is assessed as described in experimental method section. (C) Fibroblasts pretreated with RU486 (10 µM) for 30 min were incubated with G75 (5 µM) for another 24 h. (D) Fibroblasts pre-treated with GR siRNA (10 nM) or control siRNA (10 nM) for 48 h were incubated with DMSO, or G75 (5 µM) for another 24 h. All experiments were carried out in triplicate. The results are presented as means ± S.E.M (n = 3). ** p < 0.01, *** p < 0.001, two-tailed Student’s t-test.