Table 2.
Protocol | SampleID | Direct | Inverted | Insertion | Chimeric Rate (%) |
---|---|---|---|---|---|
MDA 1 | MDA1 | 585,997 | 6,363,739 | 17,425,803 | 6.31 |
MDA | MDA2 | 521,820 | 5,376,608 | 11,582,554 | 6.62 |
scMDA 2 | BGIYH1 | 240,456 | 1,763,085 | 3,334,791 | 2.37 |
scMDA | BGIYH2 | 64,154 | 598,660 | 1,600,853 | 1.42 |
scMDA | HUMDA | 2,708,326 | 4,559,919 | 4,397,551 | 3.24 |
scMDA | Qiagen1 | 167,175 | 2,206,328 | 157,692 | 0.93 |
scMDA | Qiagen5 | 227,852 | 2,867,365 | 163,770 | 1.17 |
scMDA | Qiagen9 | 224,273 | 2,993,157 | 234,983 | 1.16 |
scMDA | GE2 | 3,336,137 | 10,315,253 | 310,998 | 4.38 |
scMDA | GE4 | 5,112,524 | 12,681,862 | 323,529 | 4.24 |
scMDA | GE10 | 4,844,590 | 8,899,910 | 387,391 | 4.68 |
1 MDA: conventional phi29DNApol-mediated MDA; 2 scMDA: single cell MDA, the genomics DNA from a single cell amplified by using phi29DNApol-mediated MDA reactions.