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. 2019 May 13;12:29. doi: 10.1186/s13072-019-0276-7

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Meiosis defects are associated with altered H3K4me3 and H4Ac occupancy. a Surface spreads from E15.5 ovaries in zygotene stage and somatic cells from control (first row) and CD-exposed mice (second row) were immunostained with anti-H3K4me3 (red) and anti-SYCP3 (green) antibodies (63X magnification). c Surface spreads from E15.5 ovaries in zygotene stage and somatic cells from control and CD-exposed mice were immunostained with anti-H4 (red) and anti-SYCP3 (green) antibodies. b, d Quantitative analysis of anti-H3K4me3 and H4Ac intensities in zygotene stages of oocytes and in somatic cells. The analysis was performed on at least 20 oocytes from four replicates for each group, n = 4 for each group, *p < 0.05, **p < 0.01, ***p < 0.001, t test