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. 2019 May 14;12:122. doi: 10.1186/s13068-019-1462-3

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characterization of transformants of CrCAO. a PCR detection of the inserted plasmid (pNsChlb) by amplifying the 375 bp Shble sequence only in transformants NsChlb7 and NsChlb19. The 380 bp product of 18S rDNA was used as a loading control and was detected in all samples. b Western blotting for expression of the FLAG-tagged CrCAO protein from transformants. The Flag tag was detected only in transformants, while the β subunit of ATP synthase (Atpβ) appeared as the F-type H-ATPase β subunit (72.6 kDa) and the CF1 β subunit (53.13 kDa) in WT and transformants. c Detection of chlorophylls and other photosynthetic pigments via HPLC at 460 nm absorbance. Peaks for chlorophyll a and a few carotenoids including β-carotene and violaxanthin were shown. Emergence of chlorophyll b was detected only in the transformants