Table 1.
Photosynthetic pigments analyzed by HPLC
| (Scale: μg/mg) | Pigments | ML (90 μE) | HL (200 μE) | ||||
|---|---|---|---|---|---|---|---|
| WT | NsChlb 7 | NsChlb 19 | WT | NsChlb 7 | NsChlb 19 | ||
| Day 8 | Chla | 45.36 ± 3.13 | 57.61 ± 1.69* | 50.09 ± 1.09 | 43.92 ± 3.42 | 50.70 ± 2.30 | 39.48 ± 2.44 |
| Chlb | n.d. | 0.28 ± 0.05 | 1.51 ± 0.13 | n.d. | 0.18 + 0.01 | 0.53 + 0.07 | |
| Violaxanthin | 3.88 ± 0.06 | 4.48 ± 0.09 | 4.04 ± 0.17 | 4.10 ± 0.29 | 5.06 ± 0.26* | 3.90 ± 0.21 | |
| β-carotene | 4.42 ± 0.45 | 4.07 ± 0.83 | 4.58 ± 0.93 | 1.08 ± 0.42 | 2.36 ± 0.06** | 1.85 ± 0.07* | |
| Zeaxanthin | 0.09 ± 0.01 | 0.12 ± 0.01 | 0.16 ± 0.01 | 0.10 ± 0.01 | 0.11 ± 0.01 | 0.10 ± 0.01 | |
| Day 12 | Chla | 72.52 ± 1.90 | 75.86 ± 8.84 | 70.55 ± 4.43 | 23.25 ± 1.30 | 25.86 ± 1.16 | 20.23 ± 1.30 |
| Chlb | n.d. | 0.15 ± 0.07 | 0.97 ± 0.40 | n.d. | 0.05 ± 0.02 | 0.13 ± 0.05 | |
| Violaxanthin | 7.37 ± 0.08 | 7.52 ± 1.22 | 7.42 ± 0.29 | 1.81 ± 0.19 | 2.10 ± 0.23 | 1.70 ± 0.17 | |
| β-carotene | 5.68 ± 0.09 | 5.84 ± 0.55 | 5.49 ± 0.19 | 1.39 ± 0.10 | 1.67 ± 0.17 | 1.34 ± 0.02 | |
| Zeaxanthin | 0.12 ± 0.01 | 0.11 ± 0.01 | 0.09 ± 0.20 | 0.17 ± 0.01 | 0.20 ± 0.03 | 0.13 | |
The data represent the average value with ranges of standard error (n = 3). Significant differences between WT and each transformants (WT vs. NsChlb7, WT vs. NsChlb19) are determined by Student’s t test and are indicated by asterisks (*P < 0.05, **P < 0.01, ***P < 0.001)