Fig. 2.

Dynamic behavior during early activation of ER. ChIP-qPCR of the TFF1 gene (a) at three time points shows increased binding of ER at 45 min after MCF7 cells are stimulated by estradiol. The previously reported maximum is followed by a decrease in the TFF1 promoter occupancy at 90 min. p values are generated by one-tailed t test. The maximal point at 90 min was identified as an outlier (> median + 2 × IQR); however, the removal did not alter the significance of results. (b) Differential binding analysis of ChIP-seq data at three time points to monitor the activation of ER. The ER exhibits a strong increase in binding at 45 min vs 0 min (c), and the majority of sites still display binding at 90 min