Table-1.
Quantification of IL-4, IL-10, IFN-γ, and TNF-α cytokine in PBMCs culture supernatants by ELISA.
| Cytokines in culture supernatants | Rec-IL-4 (500 ng/ml) | Rec-IL-10 (500 ng/ml) | Con A | PHA | Control | Sham control |
|---|---|---|---|---|---|---|
| IL-4 (pg/ml) | NT | 134.1±1.15 | 212.6±3.4# | 144.6±2.49# | 31.65±1.04 | 39.15±2.35 |
| IL-10 (pg/ml) | 278.7±4.50# | NT | 113.8±2.58# | 134.0±2.50# | 36.10±1.14 | 27.60±1.60 |
| TNF-α (pg/ml) | 196.6±2.50# | 201.10±2.22# | 194.6±2.45# | 198.9±2.32# | 32.22±1.23 | 36.35±2.66 |
| IFN-γ (pg/ml) | 65.68±3.55 | 173.7±4.75# | 113.4±2.38# | 135.0±2.26# | 36.45±1.10 | 35.89±2.34 |
Results obtained at 500 ng/ml concentration of recombinant IL-4 and IL-10 are shown here. Values were expressed as mean±SEM.
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Indicating p-value having significant difference p<0.001. NT=Not tested, IL=Interleukin, IFN-γ=Interferon gamma. TNF-α=Tumor necrosis factor-alpha, PBMCs=Peripheral blood mononuclear cells, ELISA=Enzyme-linked immunosorbent assay