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. 2019 May 7;10:977. doi: 10.3389/fmicb.2019.00977

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Copyright © 2019 Zhang, Wu, Zhu, Yang, Yu, Wang and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LGG Pretreatment induced the expansion CD3^-CD19^-T-bet⁺IFNγ⁺ and CD3^-CD19^-T-bet⁺IFNγ^- cell subsets in the peripheral blood. Peripheral blood samples were collected from the indicated pigs at 0, 24, and 168 h after S. 4,[5],12:i:- challenge. (A) Representative dot plots show the percentages of T-bet^+/- IFNγ^+/- cell subsets among CD3^-CD19^- cells at 24 h after S. 4,[5],12:i:- challenge. Flow cytometry analysis of the percentages of T-bet^- IFNγ⁺ (B), T-bet⁺ IFNγ⁺ (C), and T-bet⁺ IFNγ^- cells (D) within the peripheral CD3^-CD19^- cell populations in the indicated pigs. The data are presented as mean ± SEM (n = 6 per group). ^∗P < 0.05.