Table 3.
Binding affinities of 1-hexanol and its analogs to rGmolCSP8 Wild-Type and mutants.
| Ligands |
Ki (μM) |
||||
|---|---|---|---|---|---|
| Wild-Type | T27A | L30A | V40A | L80A | |
| 1-butanol | 6.65 ± 0.52 | – | 17.09 ± 1.05 | 8.07 ± 0.61 | 6.75 ± 0.57 |
| 1-pentanol | 8.18 ± 0.93 | – | – | 12.36 ± 1.41 | 6.66 ± 0.64 |
| 1-hexanol | 2.11+0.32 | – | 12.51 ± 1.12 | 2.67 ± 0.34 | 1.80 ± 0.15 |
| 1-heptanol | 6.32 ± 0.52 | 9.01 ± 0.90 | – | 9.68 ± 0.75 | 6.02 ± 0.66 |
| 1-decanol | 6.67 ± 0.54 | 12.67 ± 1.79 | – | 7.02 ± 0.47 | 6.32 ± 0.65 |
Ki, dissociation constant. The values are the means of three independent experiments. “–” means the IC50 and Ki values were not calculated in the range of concentration ranges of tested ligands (up to 24 μM).