Figure 3 .

m⁶A modification of MTCH2 promotes adipogenesis in intramuscular preadipocytes by enhancing protein expression. A) Synonymous mutations in the MTCH2 CDS. B) Real-time qPCR analysis of immunoprecipitated m⁶A in 293T cells using MTCH2 PCR primer for amplification. C) Oil Red O staining of intramuscular preadipocytes 6 d after MDI induction (upper-left corner, high magnification). D) Relative absorption of Oil Red O (ORO) staining. E) Triglyceride content. F) Real-time qPCR analysis of adipogenic genes. G) Flag and MTCH2 protein expression in vector, MTCH2-WT, and MTCH2-Mut groups after 1 d of MDI induction. Each column represents the means ± sem. Ns, not significant. *P < 0.05, **P < 0.01 (1-way ANOVA).