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. 2018 Oct 19;33(2):2971–2981. doi: 10.1096/fj.201801393RRR

Figure 4 .

Figure 4

MTCH2 CDS mediates translation via a YTHDF1-dependent pathway. A) MTCH2 protein levels after FTO overexpression for 48 h (MD-d0) or MDI-induced for 24 h (DM-d1). B) Half-life of MTCH2 mRNA in cells transfected with MTCH2-WT vs. MTCH2-Mut. C) MTCH2 protein levels in MTCH2-WT or MTCH2-Mut with YTHDF1 knockdown after MDI induction for 24 h. D) Oil Red O staining of cell samples from C after adipogenic induction for 8 d. E) MTCH2 protein expression after YTHDF1 overexpression for 48 h (MD-d0) or MDI induction for 24 h (DM-d1). F) Western blot analysis of YTHDF1 in input and FLAG-RIP groups. G) Real-time qPCR analysis of relative MTCH2 expression levels in input and FLAG-RIP groups. Each column represents the mean ± sem. Ns, not significant. *P < 0.05, **P < 0.01.