Figure 3.

Nicotinamide riboside (NR) restores the colony formation in aged mice. (a) Isolated young or old crypts were cultured in medium with or without 1 mM NR as indicated (5–6 wells/group). Representative images of the formed organoids at day 5 and the quantification of organoids number. Original magnifications: ×50. Scale bar: 100 µm. (b) Isolated old crypts were cultured in medium with or without 1 mM NR and 1 mM rapamycin as indicated (five wells/group). (c) Isolated old crypts were cultured in medium with or without 1 mM NR and 1 µM EX527 as indicated (4–5 wells/group). (d) Isolated old intestinal stem cells (ISCs) were cultured in medium with or without 1 mM NR and 1 mM rapamycin as indicated (four wells/group). (e) Isolated old ISCs were cultured in medium with or without 1 mM NR and 1 µM EX527 as indicated (three wells/group). Values represent the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; t test