Table 1.
Body weight, body mass distribution, and serum parameters measured in 3‐m, 12‐m, 12mCR, 20‐m, and 20mCR mice
| 3 m | 12 m | 12mCR | 20 m | 20mCR | |
|---|---|---|---|---|---|
| Body weight (g) | 22.36 ± 0.42 | 34.10 ± 0.91*** | 27.25 ± 0.59### | 25.39 ± 0.72**§§§ | 20.67 ± 0.33† |
| scWAT (g) | 0.14 ± 0.01 | 0.33 ± 0.007** | 0.22 ± 0.02# | 0.23 ± 0.02**§§ | 0.30 ± 0.02 |
| eWAT (g) | 0.18 ± 0.02 | 0.48 ± 0.02** | 0.27 ± 0.03## | 0.26 ± 0.05§§ | 0.14 ± 0.03 |
| BAT (g) | 0.09 ± 0.006 | 0.18 ± 0.02** | 0.11 ± 0.01# | 0.07 ± 0.003§§§ | 0.07 ± 0.004 |
| Liver (g) | 1.05 ± 0.07 | 1.37 ± 0.08* | 1.07 ± 0.04## | 1.09 ± 0.11 | 0.76 ± 0.09 |
| Pancreas (g) | 0.22 ± 0.02 | 0.30 ± 0.01* | 0.29 ± 0.02 | 0.17 ± 0.009§§ | 0.14 ± 0.01 |
| Kidney (g) | 0.14 ± 0.01 | 0.24 ± 0.005*** | 0.19 ± 0.003### | 0.36 ± 0.01***§§§ | 0.29 ± 0.0003† |
| Soleus muscle (mg) | 7.3 ± 1.64 | 6.46 ± 0.79 | 10.92 ± 1.70 | 8.3 ± 1.1 | 8.7 ± 1.14 |
| Gastrocnemius muscle (g) | 0.09 ± 0.005 | 0.13 ± 0.005** | 0.10 ± 0.008# | 0.19 ± 0.006**§§ | 0.19 ± 0.007 |
| Fasting glucose (mg/dl) | 98.25 ± 5.40 | 100.80 ± 4.07 | 120.4 ± 9.66# | 130.8 ± 8.1**§§ | 100.8 ± 6.5† |
| Fasting insulin (µg/L) | 0.22 ± 0.11 | 0.61 ± 0.06* | 0.15 ± 0.05# | 0.62 ± 0.20 | 0.49 ± 0.10 |
| Nonfasting glucose (mg/dl) | 133.20 ± 6.46 | 129.00 ± 5.56 | 108.98 ± 5.46# | 122.00 ± 10.19 | 105.6 ± 5.23 |
| Nonfasting insulin (µg/L) | 0.50 ± 0.08 | 1.07 ± 0.13** | 0.72 ± 0.14 | 0.90 ± 0.26 | 0.30 ± 0.03 |
| HOMAIR | 1.51 ± 0.34 | 4.38 ± 0.15** | 1.29 ± 0.20### | 5.74 ± 0.94** | 5.61 ± 1.31 |
| Nonfasting leptin (ng/ml) | 4.82 ± 0.83 | 7.97 ± 2.02 | 1.91 ± 0.24# | 2.84 ± 0.53 | 2.77 ± 0.57 |
| Nonfasting MCP−1 (pg/ml) | 77.43 ± 5.85 | 200.70 ± 52.74* | 67.49 ± 17.59# | 123.20 ± 10.78** | 80.95 ± 20.94 |
| Nonfasting adiponectin (µg/ml) | 13.40 ± 1.08 | 5.57 ± 0.47* | 13.61 ± 2.53# | ‐ | ‐ |
| Serum thyroxine (T4) (ng/ml) | 45.5 ± 3.8 | 31.6 ± 4.4 | 36.9 ± 4.1 | ‐ | ‐ |
| Serum triiodothyronine (T3) (ng/ml) | 0.12 ± 0.03 | 0.09 ± 0.02* | 0.19 ± 0.04# | ‐ | ‐ |
| Daily food intake (g) | 3.92 ± 0.12 | 3.96 ± 0.10 | 3.17 ± 0.02 | ‐ | ‐ |
| Daily food intake relative to BW | 0.145 ± 0.004 | 0.113 ± 0.002* | 0.117 ± 0.0007 | ‐ | ‐ |
Data are represented as average ± SEM (n = 3–10 animals/group).
*p < 0.05,**p < 0.01,***p < 0.01 12 m or 20 m vs. 3 m;§ p < 0.05,§§ p < 0.01, §§§ p < 0.001 20 m vs. 12 m;# p < 0.05,## p < 0.01,### p < 0.001 12mCR vs. 12 m;† p < 0.05 20mCR vs. 20 m.