Figure 3.

Ang‐(1‐7) attenuates the endothelial senescent pro‐inflammatory phenotype. The levels of (a, d) ICAM‐1 and (b, e) VCAM‐1 were assessed by flow cytometry in HUVEC cultures treated for 18 h with Ang II (100 nM) or IL‐1β (2.5 ng/ml), respectively, either alone or in the presence of Ang‐(1‐7) (100 nM). In some experiments, the Mas receptor antagonist A779 (1 μM) was added. (c, f) Adhesion of human mononuclear cells to HUVEC monolayers exposed to the treatments described above. (g, h) IL‐6 secretion in supernatants from HUVEC exposed to the treatments described above. n = 3–8. *p ≤ 0.05 vs. control untreated cells, ^# p ≤ 0.05 vs. Ang II‐ or IL‐1β‐stimulated cells