Figure 1.

Genetic manipulation of PKA activity modulates pH_c upon glucose depletion. Mutants with overactive PKA activity (green) have reduced pH_c and low PKA activity mutants (red) an increased pH_c when glucose is depleted from the media. (a) BY4741 (black), bcy1Δ (green) and DAmP CYR1 (red) strains were grown in microplates and OD₆₀₀ (filled symbols) and pH_c (open symbols) were monitored. A representative example is shown. (b) and (c) summarise pH_c during exponential growth (b) and after glucose depletion (c) for the set of PKA mutants analysed (See Materials and Methods for details). Representative OD₆₀₀ and pH_c curves for each strain can be found in Supporting information Figure S1. Data represent average ± standard deviation (SD) of at least three biological replicates per strain