Figure 3.

Manipulation of PKA activity before glucose depletion modulates pH_c upon glucose starvation. (a, c) Exponentially growing BY4741 (a) or TPK1^astpk2∆tpk3∆ (c) cultures were subjected to the treatments indicated in the adjacent table and transferred to a microplate to monitor pH_c as described in Materials and Methods. Data show a representative result. Pre‐treatments indicate incubation for 90 min prior to starvation with 20 mM cAMP (a) or 2 µM 1NM‐PP1 (c), while cAMP during starvation in (a) indicates the cells were washed with and starved in media without glucose containing 20 mM cAMP. (b, d) pH_c 60 min after starvation for the indicated treatments as in (a) or (c), respectively. Data show averages ±SD of three biological replicates. Significance was tested using two‐way ANOVA with matching for both factors and Bonferroni's multiple comparisons test