Figure 5.

Transcriptional expression of fzy‐1/cdc‐20 is a target of aging and STS stress. (a) RNA‐seq analysis of expression levels of APC/C subunits in D4 N2 adult male worms starved for 2 days. Data represent mean ± SD, n = 3. (b) RNA‐seq analysis of expression levels of APC/C subunits in D4 N2 adult male worms starved for 2 days (D4S2). The ratio of mRNA levels from starved worms (D4S2) over levels before STS stress treatment (D4) was calculated. Data represent mean ± SD, n = 3. (c) qRT‐PCR analysis of mRNA levels of APC/C subunits in testes isolated from D10 adult males previously subjected to an STS stress treatment as shown in Figure 1a. qRT‐PCR analysis of the indicated genes was normalized against sgo‐1 expression levels. Data represent mean ± SD, n = 4. Difference between two indicated groups: *, p ≤ 0.05. **, p ≤ 0.01