Table 2.

Total sperm counts in RNAi‐treated male C. elegans at day 15 that were previously subjected to an STS stress

RNAi‐treated males (at 15°C)	Total sperm counts (mean ± SD, n = 8–12)					Required for STS stress‐preserved spermatogenesis
	D4	D15
		F	MS	YS	2S
Nontargeting controls
OP50 bacteria	2,405 ± 413	1,648 ± 395	1,545 ± 315	2,225 ± 497a , **	2,508 ± 458***
L4440 vector	1,900 ± 236	1,661 ± 221	1,959 ± 311*	2,479 ± 307***	2,423 ± 484**
Targeting controls
mat‐3	2,886 ± 608	2,398 ± 314	1,914 ± 251**	1,705 ± 354**	1,443 ± 492***	Yes
fzr‐1	2,406 ± 406	3,281 ± 455	2,944 ± 378	2,564 ± 381*	2,470 ± 392**	Yes
apc‐10	2,660 ± 550	1,579 ± 563	1,799 ± 514	2,002 ± 466*	2,659 ± 475***	No
Individually targeted genes
apc‐2	2,693 ± 470	1,777 ± 493	1,566 ± 639	2,025 ± 318	2,282 ± 460*	No
apc‐11	2,559 ± 481	1,219 ± 238	1,361 ± 291	1,717 ± 470*	1,799 ± 428**	No
gfi‐3	2,013 ± 432	1,448 ± 360	1,406 ± 218	1,820 ± 290*	1,986 ± 487*	No
Such‐1	2,211 ± 432	1,383 ± 295	1,260 ± 259	1,690 ± 309*	2,066 ± 365*	No
mat‐4	2,524 ± 441	2,711 ± 574	2,542 ± 334	2,409 ± 413	2,492 ± 306	Yes
apc‐17	2,312 ± 386	2,544 ± 227	2,472 ± 169	2,394 ± 142	2,421 ± 271	Yes
fzy‐1	2,300 ± 280	2,272 ± 312	2,198 ± 357	2,333 ± 311	2,325 ± 226	Yes
Multi‐targeted genes
apc‐2; apc‐10; apc‐11	2,135 ± 384	1,886 ± 430	2,061 ± 448	2,098 ± 492	2,117 ± 322	Yes
gfi‐3;such‐1	2,358 ± 252	2,008 ± 367	2,054 ± 298	2,320 ± 329	2,237 ± 264	Yes

D4: day 4; F: fed control; MS: starvation at mid‐stage; YS: starvation at early stage; 2S: twice starvation.

^aTotal sperm counts are presented as mean ± SD, n = 10–15.

^* p < 0.05,

^** p < 0.01,

^*** p < 0.001, two‐tailed Student's t test, compared to the F group of D15 worms of the same strain.