Figure 4.

Consumption of H₂O₂ by untreated skeletal muscle (a–d), and heart (e) mitochondria after exogenous addition of 2.5 µM H₂O₂. Negative values represent net consumption, positive values represent net production (see Munro et al., 2016 for methodological details). Aura = auranofin, an inhibitor of the thioredoxin reductase. For mouse skeletal muscle, n = 4 for all conditions, except for no substrate and M at 37°C, where n = 6. For NMR skeletal muscle at 37°C, n = 3; at 30°C, n = 4 for MGS +ADP, n = 5 for MGS and MGS + Aura, and n = 6 for all other conditions. For the heart, n = 4 (4 animals per preparation) for all. Other details are as in Figure 2