Figure 1.

KIN‐4 is a PDZ protein that is required for the longevity of daf‐2/insulin/IGF‐1 receptor mutants. (a) A plot of percent changes in mean lifespan of rrf‐3(pk1426) [rrf‐3(−)] and rrf‐3(pk1426); daf‐2(e1370) [rrf‐3(−); daf‐2(−)] mutants upon knocking down each candidate PDZ protein‐encoding gene. Orange circles indicate the lifespan results after knocking down each targeted gene. Blue circles indicate the effects of kin‐4 RNAi and gipc‐1/‐2 RNAi on lifespan. A gray circle indicates the lifespan decrease by daf‐16 RNAi that was used as a positive control. Error bars represent standard error of mean (SEM) of two independent lifespan experiments for each RNAi clone. (b, c) Knock‐down of kin‐4 (b) or gipc‐1/‐2 (c) had a larger lifespan‐decreasing effect on rrf‐3(−); daf‐2(−) mutants than on rrf‐3(−) animals. See Supporting information Table S2 for statistics and additional repeats